Review



sr bi sirna  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91

    Structured Review

    Santa Cruz Biotechnology sr bi sirna
    Role of SR-B I and cholesterol efflux in the anti-inflammatory properties of Por-NPs in macrophages . iBMDMs were transfected with SR-BI <t>siRNA</t> (100 nM) to knockdown SR-BI. RT-qPCR measurement of (A) Il1b mRNA and (B) Ccl5 mRNA following incubation with discoidal Por-NPs and stimulation with LPS (10 ng/mL) (n = 3 biological replicates). iBMDMs were pre-incubated with PBS, methyl-β-cyclodextrin (MβCD, 4 % v/v), discoidal or CO-loaded Por-NPs (10 μg/mL) then stimulated with LPS (10 ng/mL) before RT-qPCR quantification of the mRNA levels of (C) Il1b and (D) Ccl5 . The nuclear fractions of (E) unstimulated and (F) LPS-stimulated iBMDMs were isolated and nuclear p65-NFκB protein levels (∼65 kDa) were assessed by Western blotting (n = 5–6 biological replicates). TATA binding protein (TBP, 37 kDa) was used as the nuclear protein loading control. Data expressed as Mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 by one-way ANOVA with post-hoc Tukey's multiple comparisons.
    Sr Bi Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sr+bi+sirna/SR-B1+siRNA/pmc12398886-65-18-21
    Average 91 stars, based on 5 article reviews
    sr bi sirna - by Bioz Stars, 2026-09
    91/100 stars

    Images

    1) Product Images from "Theranostic porphyrin nanoparticles identify atherosclerosis via multimodal imaging and elicit atheroprotective effects"

    Article Title: Theranostic porphyrin nanoparticles identify atherosclerosis via multimodal imaging and elicit atheroprotective effects

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2025.102202

    Role of SR-B I and cholesterol efflux in the anti-inflammatory properties of Por-NPs in macrophages . iBMDMs were transfected with SR-BI siRNA (100 nM) to knockdown SR-BI. RT-qPCR measurement of (A) Il1b mRNA and (B) Ccl5 mRNA following incubation with discoidal Por-NPs and stimulation with LPS (10 ng/mL) (n = 3 biological replicates). iBMDMs were pre-incubated with PBS, methyl-β-cyclodextrin (MβCD, 4 % v/v), discoidal or CO-loaded Por-NPs (10 μg/mL) then stimulated with LPS (10 ng/mL) before RT-qPCR quantification of the mRNA levels of (C) Il1b and (D) Ccl5 . The nuclear fractions of (E) unstimulated and (F) LPS-stimulated iBMDMs were isolated and nuclear p65-NFκB protein levels (∼65 kDa) were assessed by Western blotting (n = 5–6 biological replicates). TATA binding protein (TBP, 37 kDa) was used as the nuclear protein loading control. Data expressed as Mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 by one-way ANOVA with post-hoc Tukey's multiple comparisons.
    Figure Legend Snippet: Role of SR-B I and cholesterol efflux in the anti-inflammatory properties of Por-NPs in macrophages . iBMDMs were transfected with SR-BI siRNA (100 nM) to knockdown SR-BI. RT-qPCR measurement of (A) Il1b mRNA and (B) Ccl5 mRNA following incubation with discoidal Por-NPs and stimulation with LPS (10 ng/mL) (n = 3 biological replicates). iBMDMs were pre-incubated with PBS, methyl-β-cyclodextrin (MβCD, 4 % v/v), discoidal or CO-loaded Por-NPs (10 μg/mL) then stimulated with LPS (10 ng/mL) before RT-qPCR quantification of the mRNA levels of (C) Il1b and (D) Ccl5 . The nuclear fractions of (E) unstimulated and (F) LPS-stimulated iBMDMs were isolated and nuclear p65-NFκB protein levels (∼65 kDa) were assessed by Western blotting (n = 5–6 biological replicates). TATA binding protein (TBP, 37 kDa) was used as the nuclear protein loading control. Data expressed as Mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 by one-way ANOVA with post-hoc Tukey's multiple comparisons.

    Techniques Used: Transfection, Knockdown, Quantitative RT-PCR, Incubation, Isolation, Western Blot, Binding Assay, Control

    Related Articles

    Control:

    Article Title: Farnesoid X receptor agonist GW4064 indirectly inhibits HCV entry into cells via down-regulating scavenger receptor class B type I.
    Article Snippet: Author’s Accepted Manuscript Farnesoid X receptor agonist GW4064 indirectly inhibits HCV entry into cells via down-regulating scavenger receptor class B type I Zhou-Yi Wu, Hu Li, Jian-Rui Li, Xiao-Qin Lv, Jian-Dong Jiang, Zong-Gen Peng PII: S0014-2999(19)30193-1 DOI: https://doi.org/10.1016/j.ejphar.2019.03.033 Reference: EJP72291 To appear in: European Journal of Pharmacology Received date: 25 November 2018 Revised date: 8 March 2019 Accepted date: 18 March 2019 Cite this article as: Zhou-Yi Wu, Hu Li, Jian-Rui Li, Xiao-Qin Lv, Jian-Dong Jiang and Zong-Gen Peng, Farnesoid X receptor agonist GW4064 indirectly inhibits HCV entry into cells via down-regulating scavenger receptor class B type I , European Journal of Pharmacology, https://doi.org/10.1016/j.ejphar.2019.03.033 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form.

    Article Title: Theranostic porphyrin nanoparticles identify atherosclerosis via multimodal imaging and elicit atheroprotective effects
    Article Snippet: Secreted proteins were measured in cell culture supernatants from treated iBMDMs and in mouse plasma using ELISAs for IL-1β, CCL5 and CCL17 (Quantikine®, R&D systems). .. iBMDMs were incubated with transfection reagent alone (Lipofectamine® RNAiMAX, 13778150, Thermofisher), scrambled control siRNA (sc-37007, Santa Cruz) or SR-BI siRNA (sc-44753, Santa Cruz) in optiMEM at 100 nM of siRNA for 6 h at 37oC. ..

    Article Title: Theranostic porphyrin nanoparticles identify atherosclerosis via multimodal imaging and elicit atheroprotective effects
    Article Snippet: Secreted proteins were measured in cell culture supernatants from treated iBMDMs and in mouse plasma using ELISAs for IL-1β, CCL5 and CCL17 (Quantikine®, R&D systems). .. iBMDMs were incubated with transfection reagent alone (Lipofectamine® RNAiMAX, 13778150, Thermofisher), scrambled control siRNA (sc-37007, Santa Cruz) or SR-BI siRNA (sc-44753, Santa Cruz) in optiMEM at 100 nM of siRNA for 6 h at 37 o C. Media was replaced and cells incubated for a total of 48 h iBMDMs were incubated with PBS or discoidal Por-NPs (10 μg/mL, R4F concentration, 24 h) then stimulated with LPS for 16 h for inflammatory gene assessments. ..

    Article Title: Multifunctional biomimetic porphyrin-lipid nanoparticles – novel nanoscale theranostics for atherosclerotic cardiovascular disease
    Article Snippet: Secreted proteins were measured in cell culture supernatants from treated iBMDMs using ELISAs for IL-1β and CCL5 (Quantikine®, R&D systems). .. iBMDMs were incubated with transfection reagent alone (Lipofectamine® RNAiMAX, 13778150, Thermofisher), scrambled control siRNA (sc-37007, Santa Cruz) or SR-BI siRNA (sc-44753, Santa Cruz) in optiMEM at 100 nM of siRNA for 6h at 37°C. ..

    Incubation:

    Article Title: Theranostic porphyrin nanoparticles identify atherosclerosis via multimodal imaging and elicit atheroprotective effects
    Article Snippet: Secreted proteins were measured in cell culture supernatants from treated iBMDMs and in mouse plasma using ELISAs for IL-1β, CCL5 and CCL17 (Quantikine®, R&D systems). .. iBMDMs were incubated with transfection reagent alone (Lipofectamine® RNAiMAX, 13778150, Thermofisher), scrambled control siRNA (sc-37007, Santa Cruz) or SR-BI siRNA (sc-44753, Santa Cruz) in optiMEM at 100 nM of siRNA for 6 h at 37oC. ..

    Article Title: Theranostic porphyrin nanoparticles identify atherosclerosis via multimodal imaging and elicit atheroprotective effects
    Article Snippet: Secreted proteins were measured in cell culture supernatants from treated iBMDMs and in mouse plasma using ELISAs for IL-1β, CCL5 and CCL17 (Quantikine®, R&D systems). .. iBMDMs were incubated with transfection reagent alone (Lipofectamine® RNAiMAX, 13778150, Thermofisher), scrambled control siRNA (sc-37007, Santa Cruz) or SR-BI siRNA (sc-44753, Santa Cruz) in optiMEM at 100 nM of siRNA for 6 h at 37 o C. Media was replaced and cells incubated for a total of 48 h iBMDMs were incubated with PBS or discoidal Por-NPs (10 μg/mL, R4F concentration, 24 h) then stimulated with LPS for 16 h for inflammatory gene assessments. ..

    Article Title: Multifunctional biomimetic porphyrin-lipid nanoparticles – novel nanoscale theranostics for atherosclerotic cardiovascular disease
    Article Snippet: Secreted proteins were measured in cell culture supernatants from treated iBMDMs using ELISAs for IL-1β and CCL5 (Quantikine®, R&D systems). .. iBMDMs were incubated with transfection reagent alone (Lipofectamine® RNAiMAX, 13778150, Thermofisher), scrambled control siRNA (sc-37007, Santa Cruz) or SR-BI siRNA (sc-44753, Santa Cruz) in optiMEM at 100 nM of siRNA for 6h at 37°C. ..

    Transfection:

    Article Title: Theranostic porphyrin nanoparticles identify atherosclerosis via multimodal imaging and elicit atheroprotective effects
    Article Snippet: Secreted proteins were measured in cell culture supernatants from treated iBMDMs and in mouse plasma using ELISAs for IL-1β, CCL5 and CCL17 (Quantikine®, R&D systems). .. iBMDMs were incubated with transfection reagent alone (Lipofectamine® RNAiMAX, 13778150, Thermofisher), scrambled control siRNA (sc-37007, Santa Cruz) or SR-BI siRNA (sc-44753, Santa Cruz) in optiMEM at 100 nM of siRNA for 6 h at 37oC. ..

    Article Title: Theranostic porphyrin nanoparticles identify atherosclerosis via multimodal imaging and elicit atheroprotective effects
    Article Snippet: Secreted proteins were measured in cell culture supernatants from treated iBMDMs and in mouse plasma using ELISAs for IL-1β, CCL5 and CCL17 (Quantikine®, R&D systems). .. iBMDMs were incubated with transfection reagent alone (Lipofectamine® RNAiMAX, 13778150, Thermofisher), scrambled control siRNA (sc-37007, Santa Cruz) or SR-BI siRNA (sc-44753, Santa Cruz) in optiMEM at 100 nM of siRNA for 6 h at 37 o C. Media was replaced and cells incubated for a total of 48 h iBMDMs were incubated with PBS or discoidal Por-NPs (10 μg/mL, R4F concentration, 24 h) then stimulated with LPS for 16 h for inflammatory gene assessments. ..

    Article Title: Multifunctional biomimetic porphyrin-lipid nanoparticles – novel nanoscale theranostics for atherosclerotic cardiovascular disease
    Article Snippet: Secreted proteins were measured in cell culture supernatants from treated iBMDMs using ELISAs for IL-1β and CCL5 (Quantikine®, R&D systems). .. iBMDMs were incubated with transfection reagent alone (Lipofectamine® RNAiMAX, 13778150, Thermofisher), scrambled control siRNA (sc-37007, Santa Cruz) or SR-BI siRNA (sc-44753, Santa Cruz) in optiMEM at 100 nM of siRNA for 6h at 37°C. ..

    Concentration Assay:

    Article Title: Theranostic porphyrin nanoparticles identify atherosclerosis via multimodal imaging and elicit atheroprotective effects
    Article Snippet: Secreted proteins were measured in cell culture supernatants from treated iBMDMs and in mouse plasma using ELISAs for IL-1β, CCL5 and CCL17 (Quantikine®, R&D systems). .. iBMDMs were incubated with transfection reagent alone (Lipofectamine® RNAiMAX, 13778150, Thermofisher), scrambled control siRNA (sc-37007, Santa Cruz) or SR-BI siRNA (sc-44753, Santa Cruz) in optiMEM at 100 nM of siRNA for 6 h at 37 o C. Media was replaced and cells incubated for a total of 48 h iBMDMs were incubated with PBS or discoidal Por-NPs (10 μg/mL, R4F concentration, 24 h) then stimulated with LPS for 16 h for inflammatory gene assessments. ..



    Similar Products

    90
    Thermo Fisher sirna against sr-bi
    Sirna Against Sr Bi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sr+bi+sirna/nf%CE%BAb+p105+recombinant+polyclonal+antibody/us11840707-348-28-36
    Average 90 stars, based on 1 article reviews
    sirna against sr-bi - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    91
    Santa Cruz Biotechnology sr bi sirna
    Role of SR-B I and cholesterol efflux in the anti-inflammatory properties of Por-NPs in macrophages . iBMDMs were transfected with SR-BI <t>siRNA</t> (100 nM) to knockdown SR-BI. RT-qPCR measurement of (A) Il1b mRNA and (B) Ccl5 mRNA following incubation with discoidal Por-NPs and stimulation with LPS (10 ng/mL) (n = 3 biological replicates). iBMDMs were pre-incubated with PBS, methyl-β-cyclodextrin (MβCD, 4 % v/v), discoidal or CO-loaded Por-NPs (10 μg/mL) then stimulated with LPS (10 ng/mL) before RT-qPCR quantification of the mRNA levels of (C) Il1b and (D) Ccl5 . The nuclear fractions of (E) unstimulated and (F) LPS-stimulated iBMDMs were isolated and nuclear p65-NFκB protein levels (∼65 kDa) were assessed by Western blotting (n = 5–6 biological replicates). TATA binding protein (TBP, 37 kDa) was used as the nuclear protein loading control. Data expressed as Mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 by one-way ANOVA with post-hoc Tukey's multiple comparisons.
    Sr Bi Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sr+bi+sirna/SR-B1+siRNA/pmc12398886-65-18-21
    Average 91 stars, based on 1 article reviews
    sr bi sirna - by Bioz Stars, 2026-09
    91/100 stars
      Buy from Supplier

    90
    Thermo Fisher sirna against sr‐bi or noncoding (scrambled) sirna
    Role of SR-B I and cholesterol efflux in the anti-inflammatory properties of Por-NPs in macrophages . iBMDMs were transfected with SR-BI <t>siRNA</t> (100 nM) to knockdown SR-BI. RT-qPCR measurement of (A) Il1b mRNA and (B) Ccl5 mRNA following incubation with discoidal Por-NPs and stimulation with LPS (10 ng/mL) (n = 3 biological replicates). iBMDMs were pre-incubated with PBS, methyl-β-cyclodextrin (MβCD, 4 % v/v), discoidal or CO-loaded Por-NPs (10 μg/mL) then stimulated with LPS (10 ng/mL) before RT-qPCR quantification of the mRNA levels of (C) Il1b and (D) Ccl5 . The nuclear fractions of (E) unstimulated and (F) LPS-stimulated iBMDMs were isolated and nuclear p65-NFκB protein levels (∼65 kDa) were assessed by Western blotting (n = 5–6 biological replicates). TATA binding protein (TBP, 37 kDa) was used as the nuclear protein loading control. Data expressed as Mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 by one-way ANOVA with post-hoc Tukey's multiple comparisons.
    Sirna Against Sr‐Bi Or Noncoding (Scrambled) Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sr+bi+sirna/pmc09325382-66-18-29
    Average 90 stars, based on 1 article reviews
    sirna against sr‐bi or noncoding (scrambled) sirna - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Shanghai GenePharma sr-bi sirna#2
    Role of SR-B I and cholesterol efflux in the anti-inflammatory properties of Por-NPs in macrophages . iBMDMs were transfected with SR-BI <t>siRNA</t> (100 nM) to knockdown SR-BI. RT-qPCR measurement of (A) Il1b mRNA and (B) Ccl5 mRNA following incubation with discoidal Por-NPs and stimulation with LPS (10 ng/mL) (n = 3 biological replicates). iBMDMs were pre-incubated with PBS, methyl-β-cyclodextrin (MβCD, 4 % v/v), discoidal or CO-loaded Por-NPs (10 μg/mL) then stimulated with LPS (10 ng/mL) before RT-qPCR quantification of the mRNA levels of (C) Il1b and (D) Ccl5 . The nuclear fractions of (E) unstimulated and (F) LPS-stimulated iBMDMs were isolated and nuclear p65-NFκB protein levels (∼65 kDa) were assessed by Western blotting (n = 5–6 biological replicates). TATA binding protein (TBP, 37 kDa) was used as the nuclear protein loading control. Data expressed as Mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 by one-way ANOVA with post-hoc Tukey's multiple comparisons.
    Sr Bi Sirna#2, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sr+bi+sirna/sr+bi+sirna+2/pmc06743029-65-15-4
    Average 90 stars, based on 1 article reviews
    sr-bi sirna#2 - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Shanghai GenePharma small interfering rna (sirna) targeting sr-bi mrna (si-sr-bi)
    Overexpression of SR-BI in ccRCC. Relative <t>mRNA</t> expression of SR-BI in normal kidney and ccRCC tissues ( a ), normal kidney cell line and ccRCC cell lines ( b ) by qRT-PCR. Protein expression of SR-BI in normal kidney and ccRCC tissues ( c ), normal kidney cell line and ccRCC cell lines ( d ) by western blot. Representative images of ORO staining, HE staining and IHC analysis of SR-BI protein in normal kidney and ccRCC tissues ( e ) (original magnification ×400). * P < 0.05, ** P < 0.01
    Small Interfering Rna (Sirna) Targeting Sr Bi Mrna (Si Sr Bi), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sr+bi+sirna/small+interfering+rnas++sirnas+/pmc05778766-123-5-18
    Average 90 stars, based on 1 article reviews
    small interfering rna (sirna) targeting sr-bi mrna (si-sr-bi) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Microsynth ag small interfering sirnas targeting the porcine sr-bi and apom sequences
    Overexpression of SR-BI in ccRCC. Relative <t>mRNA</t> expression of SR-BI in normal kidney and ccRCC tissues ( a ), normal kidney cell line and ccRCC cell lines ( b ) by qRT-PCR. Protein expression of SR-BI in normal kidney and ccRCC tissues ( c ), normal kidney cell line and ccRCC cell lines ( d ) by western blot. Representative images of ORO staining, HE staining and IHC analysis of SR-BI protein in normal kidney and ccRCC tissues ( e ) (original magnification ×400). * P < 0.05, ** P < 0.01
    Small Interfering Sirnas Targeting The Porcine Sr Bi And Apom Sequences, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sr+bi+sirna/small+interfering+sirnas+targeting+the+porcine+sr+bi+and+apom+sequences/pm28315462-127-15-20
    Average 90 stars, based on 1 article reviews
    small interfering sirnas targeting the porcine sr-bi and apom sequences - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Qiagen sr-bi sirna
    Overexpression of SR-BI in ccRCC. Relative <t>mRNA</t> expression of SR-BI in normal kidney and ccRCC tissues ( a ), normal kidney cell line and ccRCC cell lines ( b ) by qRT-PCR. Protein expression of SR-BI in normal kidney and ccRCC tissues ( c ), normal kidney cell line and ccRCC cell lines ( d ) by western blot. Representative images of ORO staining, HE staining and IHC analysis of SR-BI protein in normal kidney and ccRCC tissues ( e ) (original magnification ×400). * P < 0.05, ** P < 0.01
    Sr Bi Sirna, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sr+bi+sirna/sr+bi+sirna/pm29163190-126-18-5
    Average 90 stars, based on 1 article reviews
    sr-bi sirna - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    Role of SR-B I and cholesterol efflux in the anti-inflammatory properties of Por-NPs in macrophages . iBMDMs were transfected with SR-BI siRNA (100 nM) to knockdown SR-BI. RT-qPCR measurement of (A) Il1b mRNA and (B) Ccl5 mRNA following incubation with discoidal Por-NPs and stimulation with LPS (10 ng/mL) (n = 3 biological replicates). iBMDMs were pre-incubated with PBS, methyl-β-cyclodextrin (MβCD, 4 % v/v), discoidal or CO-loaded Por-NPs (10 μg/mL) then stimulated with LPS (10 ng/mL) before RT-qPCR quantification of the mRNA levels of (C) Il1b and (D) Ccl5 . The nuclear fractions of (E) unstimulated and (F) LPS-stimulated iBMDMs were isolated and nuclear p65-NFκB protein levels (∼65 kDa) were assessed by Western blotting (n = 5–6 biological replicates). TATA binding protein (TBP, 37 kDa) was used as the nuclear protein loading control. Data expressed as Mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 by one-way ANOVA with post-hoc Tukey's multiple comparisons.

    Journal: Materials Today Bio

    Article Title: Theranostic porphyrin nanoparticles identify atherosclerosis via multimodal imaging and elicit atheroprotective effects

    doi: 10.1016/j.mtbio.2025.102202

    Figure Lengend Snippet: Role of SR-B I and cholesterol efflux in the anti-inflammatory properties of Por-NPs in macrophages . iBMDMs were transfected with SR-BI siRNA (100 nM) to knockdown SR-BI. RT-qPCR measurement of (A) Il1b mRNA and (B) Ccl5 mRNA following incubation with discoidal Por-NPs and stimulation with LPS (10 ng/mL) (n = 3 biological replicates). iBMDMs were pre-incubated with PBS, methyl-β-cyclodextrin (MβCD, 4 % v/v), discoidal or CO-loaded Por-NPs (10 μg/mL) then stimulated with LPS (10 ng/mL) before RT-qPCR quantification of the mRNA levels of (C) Il1b and (D) Ccl5 . The nuclear fractions of (E) unstimulated and (F) LPS-stimulated iBMDMs were isolated and nuclear p65-NFκB protein levels (∼65 kDa) were assessed by Western blotting (n = 5–6 biological replicates). TATA binding protein (TBP, 37 kDa) was used as the nuclear protein loading control. Data expressed as Mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 by one-way ANOVA with post-hoc Tukey's multiple comparisons.

    Article Snippet: iBMDMs were incubated with transfection reagent alone (Lipofectamine® RNAiMAX, 13778150, Thermofisher), scrambled control siRNA (sc-37007, Santa Cruz) or SR-BI siRNA (sc-44753, Santa Cruz) in optiMEM at 100 nM of siRNA for 6 h at 37 o C. Media was replaced and cells incubated for a total of 48 h iBMDMs were incubated with PBS or discoidal Por-NPs (10 μg/mL, R4F concentration, 24 h) then stimulated with LPS for 16 h for inflammatory gene assessments.

    Techniques: Transfection, Knockdown, Quantitative RT-PCR, Incubation, Isolation, Western Blot, Binding Assay, Control

    Overexpression of SR-BI in ccRCC. Relative mRNA expression of SR-BI in normal kidney and ccRCC tissues ( a ), normal kidney cell line and ccRCC cell lines ( b ) by qRT-PCR. Protein expression of SR-BI in normal kidney and ccRCC tissues ( c ), normal kidney cell line and ccRCC cell lines ( d ) by western blot. Representative images of ORO staining, HE staining and IHC analysis of SR-BI protein in normal kidney and ccRCC tissues ( e ) (original magnification ×400). * P < 0.05, ** P < 0.01

    Journal: BMC Cancer

    Article Title: Up-regulation of SR-BI promotes progression and serves as a prognostic biomarker in clear cell renal cell carcinoma

    doi: 10.1186/s12885-017-3761-z

    Figure Lengend Snippet: Overexpression of SR-BI in ccRCC. Relative mRNA expression of SR-BI in normal kidney and ccRCC tissues ( a ), normal kidney cell line and ccRCC cell lines ( b ) by qRT-PCR. Protein expression of SR-BI in normal kidney and ccRCC tissues ( c ), normal kidney cell line and ccRCC cell lines ( d ) by western blot. Representative images of ORO staining, HE staining and IHC analysis of SR-BI protein in normal kidney and ccRCC tissues ( e ) (original magnification ×400). * P < 0.05, ** P < 0.01

    Article Snippet: Small interfering RNA (siRNA) targeting SR-BI mRNA (si-SR-BI) and scrambled negative control (si-NC) was designed and synthesized by Genepharma (Shanghai, China).

    Techniques: Over Expression, Expressing, Quantitative RT-PCR, Western Blot, Staining

    Correlation between  SR-BI mRNA  expression and clinicopathological parameters of ccRCC patients

    Journal: BMC Cancer

    Article Title: Up-regulation of SR-BI promotes progression and serves as a prognostic biomarker in clear cell renal cell carcinoma

    doi: 10.1186/s12885-017-3761-z

    Figure Lengend Snippet: Correlation between SR-BI mRNA expression and clinicopathological parameters of ccRCC patients

    Article Snippet: Small interfering RNA (siRNA) targeting SR-BI mRNA (si-SR-BI) and scrambled negative control (si-NC) was designed and synthesized by Genepharma (Shanghai, China).

    Techniques: Expressing

    Knockdown of SR-BI inhibited the growth of ccRCC cells. Expression of SR-BI mRNA ( a ) and protein ( b ) was effectively inhibited by specific siRNA in ccRCC cell lines. MTT assays showed that proliferative ability of ccRCC cells transfected with si-SR-BI significantly decreased compared with control cells ( c ). Plate colony formation assays exhibited that ccRCC cells transfected with si-SR-BI formed fewer colonies than control cells ( d-e ). * P < 0.05, ** P < 0.01

    Journal: BMC Cancer

    Article Title: Up-regulation of SR-BI promotes progression and serves as a prognostic biomarker in clear cell renal cell carcinoma

    doi: 10.1186/s12885-017-3761-z

    Figure Lengend Snippet: Knockdown of SR-BI inhibited the growth of ccRCC cells. Expression of SR-BI mRNA ( a ) and protein ( b ) was effectively inhibited by specific siRNA in ccRCC cell lines. MTT assays showed that proliferative ability of ccRCC cells transfected with si-SR-BI significantly decreased compared with control cells ( c ). Plate colony formation assays exhibited that ccRCC cells transfected with si-SR-BI formed fewer colonies than control cells ( d-e ). * P < 0.05, ** P < 0.01

    Article Snippet: Small interfering RNA (siRNA) targeting SR-BI mRNA (si-SR-BI) and scrambled negative control (si-NC) was designed and synthesized by Genepharma (Shanghai, China).

    Techniques: Knockdown, Expressing, Transfection, Control